National Centre for Advanced Tribology at Southampton (nCATS), School of Engineering, University of Southampton) has expertise in developing screening bioassays for novel antifouling (AF) compounds, in order to provide more insightful AF testing directly on surfaces for both static and hydrodynamic conditions. Novel bioassay protocols have been developed to test the in situ AF efficacy of the NP on coated surfaces. This was assessed by quantifying biofilm growth and adhesion kinetics using a multidetectionmicroplate reader utilising viability staining and natural bioluminescence. Additionally, flow cells and microfluidic channels have permitted the AF performance of coatings and NPs to be explored in terms of biofilm attachment and growth for controlled hydrodynamic regimes. These bioassays were corroborated using a suite of microscopy techniques, in order to compare biofilm structures in the presence and absence of the NPs. In addition, nCATS has the capability to undertake coated panel testing using salt fog / humidity chamber (Ascott CC450xp chamber), as well as seawater field trials from a pontoon situated in a tidal basin at the National Oceanography Centre in Southampton.